These results indicate the occurrence of inter-mitochondrial complementation through exchange of genetic contents between exogenously introduced mitochondria with ΔmtDNA4696 and host mitochondria ...
The bimolecular fluorescence complementation (BiFC) assay has been widely used to examine interactions between integral and peripheral proteins within putative plasma membrane (PM) microdomains. In ...
We contend that microfluidic droplets offer a tractable platform for developing and standardizing a single-cell enzyme activity assay specifically adapted to handle the high diversity and ...
This important study investigates the role of ATG6 in regulating NPR1, a key protein in the plant immune response. The authors present compelling evidence that ATG6 not only interacts with NPR1 in ...
Modulation of Protein–Protein Interactions with Molecular Glues in a Synthetic Condensate Platform
Next to microscopic evaluation, we also developed a plate reader assay for the evaluation of molecular glues in condensates more compatible with classical high-throughput screening. Split luciferase ...
Elevated liver enzymes can occur for many reasons. Most often, elevated liver enzymes are temporary and resolve on their own without treatment. Occasionally, elevated liver enzymes require additional ...
Rubisco is the enzyme responsible for carbon fixation in plants, algae and many bacteria. A screen of a comprehensive library of rubisco variants (each with a different single-amino-acid mutation ...
Temperature affects enzyme-catalysed reactions by influencing the rate at which they occur. For a reaction to occur between an enzyme and substrate the two molecules need to collide. At lower ...
Mechanistically, luminescence complementation assays indicated that PEBP1 interacts with eIF2α, and that this interaction is reduced upon stress induced phosphorylation of eIF2α-S51. Although our ...
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