The authors then used a NanoBiT luminescence complementation assay to show the PEBP1-eIF2a interaction and its disruption by S51 phosphorylation. Strengths: Taken together, this work is novel, and the ...
By utilizing mass spectrometry-based cellular thermal shift assay (MS-CETSA), we uncovered that phosphatidylethanolamine ... Real-time interaction analysis using luminescence complementation in live ...
Modulation of Protein–Protein Interactions with Molecular Glues in a Synthetic Condensate Platform
Split luciferase complementation via stabilization of the ... as determined by conventional assays in dilute solution. This luminescence-based condensate system also allowed us to highlight the ...
The GloMax ® Discover System provides flexible use of filters and includes: high-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and ...
Developed with Promega reagents to provide a simple means of detecting advanced chemistries, the GloMax ® Explorer measures luminescence ... cell and gene reporter assays.
The applications of UCNs are not limited to bioimaging. In the following section, the various UC luminescence-based detection and sensing methods are reviewed. For some of these examples ...
gracilirostris and has been optimized to produce a luciferase enzyme subunit with improved luminescence and stability (Hall et al., 2012). BRET assays have traditionally employed GFP variants (e.g., ...
The bimolecular fluorescence complementation (BiFC) assay has been widely used to examine interactions between integral and peripheral proteins within putative plasma membrane (PM) microdomains. In ...
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